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Journal: The Journal of General Physiology
Article Title: Function of a STIM1 Homologue in C. elegans : Evidence that Store-operated Ca 2+ Entry Is Not Essential for Oscillatory Ca 2+ Signaling and ER Ca 2+ Homeostasis
doi: 10.1085/jgp.200609611
Figure Lengend Snippet: Induction of the intestinal unfolded protein response. (A) Fluorescent micrographs of control hsp-4 ∷GFP worms fed bacteria containing the empty feeding vector, control hsp-4 ∷GFP worms treated with tunicamycin, and hsp-4 ∷GFP worms fed either sca-1 or stim-1 dsRNA–producing bacteria. Worms were imaged using a stereo dissecting microscope and CCD camera (see Materials and methods). Images were obtained with identical camera settings. (B) Changes in whole worm fluorescence induced by tunicamycin (Tun), sca-1 RNAi, or stim-1 RNAi. Values are means ± SEM ( n = 275–732) and are plotted relative to fluorescence in control animals. *, P < 0.001 compared with control worms. (C) Effect of combined sca-1 and stim-1 RNAi on whole worm fluorescence. Values are means ± SEM ( n = 242–756) and are plotted relative to fluorescence in control animals. *, P < 0.0001 compared with sca-1 ( RNAi ) worms. Fluorescence levels in B and C were quantified using a COPAS BioSort and normalized to time-of-flight (i.e., GFP fluorescence/time-of-flight), which is a measure of worm size. Raw GFP fluorescence levels showed an identical pattern of change and statistical significance.
Article Snippet: Fluorescence and DIC micrographs were obtained using a
Techniques: Control, Bacteria, Plasmid Preparation, Microscopy, Fluorescence